Stefania Pasqualini

Curriculum Vitae

Date/ Place of Birth

3 February, 1958; Perugia, Italy

Nationality

Italian

Work Address

Department of Chemistry, Biology and Biotechnology - University of Perugia, Perugia, Italy
Tel: +39-075-5856413; Fax: +39-075-5856425
Email: stefania.pasqualini@unipg.it

Education

Degree in Biological Sciences, Perugia University (1982)
Habilitation for teaching Sciences in Secondary School (1982)
Habilitation for teaching Sciences in  High School (1986)
PhD (Plant Biotechnology), Perugia University (1992)
Habilitation (Full Professor) in Plant Science (2014)

Languages

Italian, English and French

Current Position  

Professor of Plant Science, Perugia University

Teaching experience

1998 – Present: Lecturer, Plant Physiology, for Biological Sciences, Perugia University
2002 – Present: Lecturer, Plant Ecophysiology, for Natural Sciences, Perugia University
2004 – 2010: Lecturer, Plant Physiology, for Biotechnology, Perugia University

Tutor/ Supervisor

Honours, Masters, and national and international PhD students

Research Experience

Long-standing experience in Plant Biochemistry and Plant Molecular Biology, with particular emphasis on plant stress responses to abiotic (ozone pollution, water stress) and biotic stress. Research activities are in the following fields: (a) Isolation, partial purification, and biochemical characterization of acid phosphatase in crop species (barley); (b) Heavy metal challenge (cadmium) in Phragmites australis, a species widely used in phytoremediation programmes: structural, ultrastructural and biochemical responses in leaves and roots; (c) Effects of biotic and abiotic stress on cell metabolism, with particular focus on photosynthetic metabolism, antioxidant enzymes, the xanthophyll cycle, carbohydrate allocation, and structural and ultrastructural leaf modifications (barley, tobacco, Arabidopsis, poplar, tomato); (d) Molecular and biochemical characterisation of nitric oxide and MAPK cascade signalling functions in plants challenged with the pollutant ozone (tobacco, Arabidopsis); (e) The role of the nitric oxide in the regulation of defence genes in plants challenged with biotic and abiotic stress.
At present Pasqualini research group is focusing on questions related to the interactions of hormonal (ethylene, jasmonic acid, salicylic acid and abscisic acid) signaling cascades and reactive oxygen species in regulating gene expression and the initiation and progression of programmed cell death and plant defense responses under biotic and abiotic stress. Modern genomics- and system biology approaches are used to study these questions. We have collaboration with several groups in Austria (Salzburg), Spain (Granada), Saudi Arabia (Thuwal) and South Africa (Western Cape) in topics relating to hormone biology, microarray expression analysis and oxidative stress.
The Pasqualini Laboratory operates in the Department of Applied Biology of Perugia University, which is an interdisciplinary expert group in the following disciplines: Plant Physiology, Plant Molecular Biology, Plant Genetics, Plant Biochemistry and Botany. The Laboratory has all the necessary equipment and facilities for high-level research into Plant Biology, such as ‘walk in’ plant growth chambers with the control of light, temperature and humidity, fumigation chambers to challenge plants with ozone, all the instrumentation necessary for spectrophotometry, high pressure liquid chromatography, ultracentrifugation, gel electrophoresis, and real-time PCR.
Prof. Pasqualini is co-author of more than 50 scientific publications in peer-reviewed international journals and books (sum of citations, 970; h-index: 18; source, ISI Web of Science 2013). Google Scholar profile: http://scholar.google.it/citations?user=EUTsTYcAAAAJ&hl=it&oi=ao.

Reviewing activities

Reviewer of manuscripts and scientific publications for e.g. Plant and Cell Environment, Environmental pollution, Plant and Cell Physiology, Plant Biology Journal of Biotechnology, Journal of Plant Growth Regulation, Plant Physiology and Agriculture, Ecosystems & Environment.

Funds and Grants

1990 - Fellow from C.I.H.E.M. (International Center for Advanced Mediterranean Agronomic Studies) for participation "Trends in Photosynthesis Research (1990)
1994 - Fellow from "British-Italian partnership program for early career researchers" to develop research activity at Department of Animal and Plant Sciences University of Sheffield (UK)
2003 - MIUR – National Interest Research Program
2003 - Private Grant from “Fondazione Cassa di Risparmio Perugia”
2005 - MIUR – National Interest Research Program
2005 -  Private Grant from “Fondazione Cassa di Risparmio Perugia”
2006 - F.I.S.R. M.I.C.E.N.A.
2012 - Private Grant from “Fondazione Cassa di Risparmio Perugia”
2012 - MIUR – National Interest Research Program
2013 - Regione Lazio

Academy and Scientific Society

Member of Doctoral School of  Biotechnology (2013)
Member of Doctoral School of Plant biology and agro environmental biotechnology (2000-2012)
Member of Scientific Committee of the “Research center on climate and climatic change” (2013)
Member of Plant Biology Society (SBI)
Member of European Societies of Plant biology
Member of Executive Committee of SBI (2011 at present)
Member of Bioethics Committee for Perugia University (20012-2016)
Biological Science (BS) course coordinator (2011 at present)
President of BS grant administration (2005 at present)
Delegate for BS course for International mobility (Erasmus and Erasmus Placement program)
Member of Paritetic Committee for BS course (2008 at present)
Referent for BS course standard quality (2013)

Selected Publications

N. M. Ordoñez, C. Marondedze, L. Thomas, S. Pasqualini, L. Shabala, S. Shabala, C. Gehring (2014) Cyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis roots. In press FEBBS LETTERS.

L. Thomas, C. Marondedze, L. Ederli, S. Pasqualini, C. Gehring (2013). Proteomic signatures implicate cAMP in light and temperature responses in Arabidopsis thaliana. JOURNAL OF PROTEOMICS, vol. 83,   p 47-59.

D. Di Baccio, L. Ederli, R. Marabottini, M. Badiani, A. Francini, C. Nali, M. Antonelli, E. Santangelo, L. Sebastiani, S. Pasqualini. (2012). Similar foliar lesions but opposite hormonal patterns in a tomato mutant impaired in ethylene perception and its near isogenic wild type challenged with ozone. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, vol. 75, p. 286-297.

S. Pasqualini, L. Reale, O. Calderini, R. Pagiotti, L. Ederli (2012). Involvement of protein kinases and calcium in the NO-signalling cascade for defence-gene induction in ozonated tobacco plants. JOURNAL OF EXPERIMENTAL BOTANY, vol. 63, p. 4485-4496.

Pasqualini S., Tedeschini E., Frenguelli G., Wopfner N., Ferreira F., D'Amato G., Ederli L. (2011). Ozone affects pollen viability and NAD(P)H oxidase release from Ambrosia artemisiifolia pollen. ENVIRONMENTAL POLLUTION, vol. 159, p. 2823-2830.

L. Ederli, L Madeo., O Calderini., C.Gehring, C.Moretti, R.Buonaurio, F. Paolocci,  S.Pasqualini  (2011). The Arabidopsis thaliana cysteine-rich receptor-like kinase CRK20 modulates host responses to Pseudomonas syringae pv. tomato DC3000 infection. JOURNAL OF PLANT PHYSIOLOGY, vol. 168, p. 1784-1794.

M. Quaglia, L. Ederli, S. Pasqualini, A. Zazzerini (2011). Biological control agents and chemical inducers of resistance for postharvest control of Penicillium expansum Link. on apple fruit. POSTHARVEST BIOLOGY AND TECHNOLOGY, vol. 59 , p. 307-315.

Pasqualini S., Meier S., Gehring C., Madeo L., Fornaciari M., Romano B., Ederli L. (2009). Ozone and nitric oxide induce cGMP-dependent and -independent transcription of defence genes in tobacco. NEW PHYTOLOGIST, vol. 181, p. 860-870.

Ederli L., Reale L., Madeo L., Ferranti F., Gehring C., Fornaciari M., Romano B., Pasqualini S. (2009). NO release by nitric oxide donors in vitro and in planta. PLANT PHYSIOLOGY AND BIOCHEMISTRY, vol. 47, p. 42-48.

Reale L., Sgromo C., Ederli L., Pasqualini S., Orlandi F., Fornaciari M., Ferranti F., Romano B. (2009). Morphological and cytological development and starch accumulation in hermaphrodite and staminate flowers of olive (Olea europaea L.). SEXUAL PLANT REPRODUCTION, vol. 22, p. 109-119.

Meier S., Madeo L., Ederli L., Donaldson L., Pasqualini S., Gehring C. (2009). Deciphering cGMP signature and cGMP-dependent pathways in plant defence. PLANT SIGNALING & BEHAVIOR, vol. 4, p. 307-309.

Villarini M, Fatigoni C, Dominici L, Maestri S, Ederli L, Pasqualini S, Monarca S, Moretti M. (2009). Assessing the genotoxicity of urban air pollutants using two in situ plant bioassays. ENVIRONMENTAL POLLUTION, vol. 157, p. 3354-3356.

Ederli L., Meier S., Borgogni A., Reale L., Ferranti F., Ghering C., Pasqualini S. (2008). cGMP in ozone and NO dependent responses. PLANT SIGNALING & BEHAVIOR, vol. 3, p. 36-37.

Antonelli M., Di Baccio D., Ederli L., Francini A., Marabottini R., Pellegrini E., Ciaffi M., Lorenzini G., Nali C., Pasqualini S., Santangelo E., Sebastiani L., Soressi G., Badiani M. (2008). Ozone as a tool for studying stress responses in tomato: signalling and defence in normal and mutant lines. ACTA HORTICULTURAE, vol. 789, p. 159-166.

Castagna A., Ederli L., Pasqualini S., Mensuali-Sodi A., Baldan B., Donnini S., Ranieri A. (2007). The tomato ethylene receptor Le-ETR3 (NR) is not involved in mediating ozone sensitivity: causal relationships among ethylene emission, oxidative burst and tissue damage. NEW PHYTOLOGIST, vol. 174, p. 342-356.

Pasqualini S., Paolocci F., Borgogni A., Morettini R., Ederli L. (2007). The overexpression of an alternative oxidase gene triggers ozone sensitivity in tobacco plants. PLANT, CELL AND ENVIRONMENT, vol. 30, p. 1545-1556.

Castagna A., Ederli L., Pasqualini S., Mensuali-Sodi A., Baldan B., Ranieri A. (2007). Role of ethylene in triggering ROS production in the tomato mutant Nr subjected to acute ozone treatment. In: - Avances in Plant Ethylene Research. Proceedings of the 7th International Symposium on the Plant Hormone Ethylene. p. 287-288, Dordrecht (The Netherlands): A. Ramina, C. Chang, J. Giovannoni, H. Klee, P. Perata, E. Woltering.

Pasqualini S., Morettini R., Borgogni A., Ederli L. (2007). Cross talk between ethylene, nitric oxide and salicylic acid in ozone fumigated tobacco plants.In: - Advances in Plant Ethylene Research. Proceedings of the 7th International Symposium on the Plant Hormone Ethylene. p. 383-385, Dordrecht (The Netherlands):A. Ramina, C. Chang, J. Giovannnoni, H. Klee, P. Perata, E. Woltering.

Antonelli M., Marabottini R., Francini A., Pellegrini E., Di Baccio D., Ederli L., Santangelo E., Nali C., Pasqualini S., Soressi G., Ciaffi M., Lorenzini G., Sebastiani L., Badiani M. (2007). Ozone as a tool for studying stress responses in tomato (Solanum lycopersicum L.). III. Ethylene, cyanide and the development of foliar symptoms in the autonecrotic mutant V20368.  In: - Advances in Plant Ethylene Research. Proceedings of the 7th International Symposium on the Plant Hormone Ethylene . p. 389-390, Dordrecht (The Netherlands):A. Ramina, C. Chang, J. Giovannoni, H. Klee, P. Perata, E. Woltering.

Fares S., Barta C., Brilli F., Centritto M., Ederli L., Ferranti F., Pasqualini S., Reale L., Tricoli D., Loreto F. (2006). Impact of high ozone on isoprene emission, photosynthesis and histology of developing Populus alba leaves directly or indirectly exposed to the pollutant. PHYSIOLOGIA PLANTARUM, vol. 128, p. 456-465.

Tosti N., Pasqualini S., Borgogni A., Ederli L., Falistocco E., Crispi S., Paolocci F. (2006). Gene expression profiles of O3-treated Arabidopsis plants. PLANT, CELL AND ENVIRONMENT, vol. 29, p. 1686-1702.

Ederli L., Morettini R., Borgogni A., Wasternack C., Miersch O., Reale L., Ferranti F., Tosti N., Pasqualini S. (2006). Interaction between nitric oxide and ethylene in the induction of alternative oxidase in ozone-treated tobacco plants. PLANT PHYSIOLOGY, vol. 142, p. 595-608.

Velikova V., Pinelli P., Pasqualini S., Reale L., Ferranti F., Loreto F. (2005). Isoprene decreases the concentration of nitric oxide in leaves exposed to elevated ozone. NEW PHYTOLOGIST, vol. 166, p. 419-426.
Ederli L., Reale L., Ferranti F., Pasqualini S. (2004). Responses induced by high concentration of cadmium in Phragmites australis roots. PHYSIOLOGIA PLANTARUM, vol. 121, p. 66-74.

Bellucci M., Ederli L., De Marchis F., Pasqualini S., Arcioni S. (2004). Transformation of Lotus corniculatus plants with E. coli asparagine synthetase A: effect on nitrogen assimilation and plant development.PLANT CELL TISSUE AND ORGAN CULTURE, vol. 78, p. 139-150.

Pasqualini S., Piccioni C., Reale L., Ederli L., Della Torre G., Ferranti F. (2003). Ozone-induced cell death in BelW3 plants: the role of programmed cell death in lesion formation. PLANT PHYSIOLOGY, vol. 133, p. 1122-1134.

Pietrini F., Iannelli M.A., Pasqualini S., Massacci A. (2003). Interaction of cadmium with glutathione and photosynthesis in developing leaves and chloroplasts of Phragmites australis (Cav.) Trin. Ex steudel. PLANT PHYSIOLOGY, vol. 133, p. 829-837.

Pasqualini S., Antonielli M., Ederli L., Piccioni C., Loreto F. (2002). Ozone uptake and its effect on photosynthetic parameters of two tobacco cultivars with contrasting ozone sensitivity. PLANT PHYSIOLOGY AND BIOCHEMISTRY, vol. 40, p. 599-603.

Pasqualini S., Della Torre G., Ferranti F., Ederli L., Piccioni C., Reale L., Antonielli M. (2002). Salicylic acid modulates ozone-induced hypersensitive cell death in tobacco plants. PHYSIOLOGIA PLANTARUM, vol. 115, p. 204-212.

Antonielli M., Pasqualini S., Batini P., Ederli L., Massacci A., Loreto F. (2002). Physiological and anatomical characterisation of Phragmites australis leaves. AQUATIC BOTANY, vol. 72, p. 55-66.

Loreto F., Mannozzi M., Maris C., Nascetti P., Ferranti F., Pasqualini S. (2001). Ozone quenching properties of isoprene and its antioxidant role in leaves. PLANT PHYSIOLOGY, vol. 126, p. 993-1000.

Pasqualini S., Batini P., Ederli L., Porceddu A., Piccioni C., De Marchis F., Antonielli M. (2001). Effects of short-term ozone fumigation on tobacco plants: response of the scavenging system and expression of the glutathione reductase. PLANT, CELL AND ENVIRONMENT, vol. 24, p. 245-252.

Pasqualini S., Ederli L., Piccioni C., Batini P., Bellucci M., Arcioni S., Antonielli M. (2001). Metabolic regulation and gene expression of root phosphoenolpyruvate carboxylase by different nitrogen sources. PLANT, CELL AND ENVIRONMENT, vol. 24, p. 439-447.

Battistelli A., Pasqualini S., Moscatello S., Ederli L., Proietti S., Antonielli M. (2001). Effects of short-term ozone fumigation on carbohydrates in darkened tobacco leaves. PLANT PHYSIOLOGY AND BIOCHEMISTRY, vol. 39, p. 539-543.

Pasqualini S., Batini P., Ederli L., Antonielli M. (1999). Responses of the xanthophyll cycle and ascorbate-glutathione cycle to ozone stress in two tobacco cultivars. FREE RADICAL RESEARCH, vol. 31, p. 67-73.

Pasqualini S., Ederli L., Batini P., Antonielli M. (1998). Effects of ozone on soluble and thylakoid proteins of two tobacco cultivars with different ozone sensitivity.. In: Responses of Plant Metabolism to Air Pollution. p. 417-421, LEIDEN:Backhuys Publishers, Leiden, The Netherlands.

Pasqualini S., Panara F., Edeli L., Batini P., Antonielli M. (1997). Multiple acid phosphatases in barley coleoptile.Isolation and partial characterization of 63 kDa soluble enzyme form.  PLANT PHYSIOLOGY AND BIOCHEMISTRY, vol. 35, p. 95-101.

Ederli L., Pasqualini S., Batini P., Antonielli M. (1997). Photoinhibition and oxidative stress: effects on xanthophyll cycle, scavenger enzymes and ABA content in tobacco plants. JOURNAL OF PLANT PHYSIOLOGY, vol. 151, p. 422-428.

Antonielli M., Pasqualini S., Ederli L., Batini P., Moscatello S., Loreto F. (1997). Physiological characteristics of tobacco cultivars with contrasting sensitivity to ozone. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, vol. 38, p. 271-277.

Pasqualini S., Batini P., Eerli L., Panara F., Antonielli M. (1996). Acid phosphatase isoforms in dry seeds and during seedling development in barley (Hordeum vulgare). CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, vol. 74, p. 653-658.

Batini P., Ederli L., Pasqualini S., Antonielli M., Valenti V. (1995). Effects of ethylenediurea and ozone in detoxificant ascorbic-ascorbate peroxidase system in tobacco plants. PLANT PHYSIOLOGY AND BIOCHEMISTRY, vol. 33, p. 717-723.

Batini P., Ederli L., Pasqualini S. (1995). The redox state of two tobacco cultivar chloroplasts following treatment with ozone. In: Photosynthesis: from light to biosphere. vol. IV, p. 409-412, Dordrecht:Kluwer Academic Publishers.

Ederli L., Pasqualini S., Batini P., Antonielli M. (1995). Variations in the xanthophyll cycle in tobacco plants in oxidative stress conditions. In: Photosynthesis: from light to biosphere. vol. IV, p. 91-94, Dordrecht:Kluwer Academic Publishers.

Pasqualini S., Batini P., Ederli L., Antonielli M. (1995). Changes induced by ozone in protein pattern of two tobacco cultivars. In: Photosynthesis: from light to biosphere. vol. IV, p. 413-416, Dordrecht:Kluwer Academic Publishers.

Pasqualini S., Panara F., Antonielli M. (1992). Acid phosphatase activity in Pinus pinea-Tuber albidum ectomycorrhizal association. CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, vol. 70, p. 1377-1383.

Pasqualini S., Lluch C., Antonielli M. (1991). Seed storage proteins in several genetic lines of Vicia faba L. PLANT PHYSIOLOGY AND BIOCHEMISTRY, vol. 29, p. 507-515, ISSN: 0981-9428.

Panara F., Pasqualini S., Antonielli M. (1990). Multiple forms of barley roots acid phosphatase: purification and some characteristics of the major cytoplasmic isoenzyme.  BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1037, p. 73-80.